Species Typing with PCR–RFLP from Cutaneous Leishmaniasis Patients in Iraq
نویسنده
چکیده
A total of 20 skin lesion samples collected from patients with cutaneous leishmaniasis (CL) attended AL-Karamah Teaching Hospital at Wasit province. Dermal scrapings were analysed by examination of Giemsa-stained smears, by Conventional PCR and RFLP-PCR methods. The incidence rate with CL was 80% by using Giemsa-stained and 100% for both PCR and RFLP-PCR. Twelve (60%) of isolates were typed as L. major (LmA & LmB) and 8 (40%) were typed as L.tropica (LtA & LtB) by using HaeIII enzyme. The higher infection (45%) appeared in age group (1-12) years old and the wet type was predominance (65%).
منابع مشابه
kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite
Background: Cutaneous Leishmaniasis, is endemically observed in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention. Microscopic identification of Leishmania species has not provided promising efficacy. The aim of this study was to determine the Le...
متن کاملSpecies Identification and Molecular Typing of Leishmania Spp. Using Targeting HSP70 Gene in Suspected Patients of Cutaneous Leishmaniasis from Sistan and Baluchestan Province, Southeast Iran
BACKGROUND Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed to detect the parasite species using the PCR-RFLP method. METHODS A total o...
متن کاملDetermination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method
Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction f...
متن کاملCytochrome b and Molecular Typing of Leishmania spp. in a Passive Sampling of Suspected Patients with Cutaneous Leishmaniasis in Sistan and Baluchestan Province, Eastern Iran
Background Despite the high prevalence and drug resistance of disease in Sistan and Baluchestan Province of Iran, the species of cutaneous leishmaniasis (CL) has not been identified. In the present study, cytochrome b (Cyt b) was used in Sistan and Baluchestan to find species of Leishmania in suspected patients of CL using PCR-RFLP and DNA sequencing. Methods This study was conducted from Oct...
متن کاملThe PCR-RFLP-Based Detection and Identification of the Leishmania Species Causing Human Cutaneous Leishmaniasis in the Khorasan-Razavi Province, Northeast of Iran
Background Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in Iran. Methods Cross-sectional study was designed to identify Leishmania species in cutaneous leishmaniasis patients who referred to Mashhad Health Centers from 2013 to 2014 using ITS-PCR-RFLP technique. Fir...
متن کامل